Job ID: 6206

Ph.D. Student in PARIS/France

Position: Ph.D. Student

Deadline: 15 May 2021

City: Paris

Country: France

Institution: Institut Pasteur

Department:

Description:

We are looking for a candidate with Master degree (five years of academic education, or equivalent from a university within or outside France) to apply for the highly competitive joint doctoral program between Institut Pasteur and Sorbonne Université:

PhD project: CRIPSR gene editing to correct audition and vision sensory deficits

Over the last years, comparative studies of the hearing and visual sensory systems enabled us to shed light on their key normal functioning & enabled an integrated view of the disease mechanisms behind hearing and vision loss in Usher syndrome, the major cause of deaf-blindness in humans (Geleoc & El-Amraoui 2020). Applicability and validation of efficient gene therapies to stop or correct Usher senses are urgently needed. The recent and constant improving gene editing tools provide novel opportunities for the treatment of rare diseases (Delmaghani & El-Amraoui 2020). The project is interdisciplinary, with cutting-edge techniques in cell biology, imaging, physiology (some benefiting from external collaborations), and CRISPR/Cas9 gene editing to enable a thorough and accurate phenotyping and treatment of available Usher models (Dulon et al. 2018). 3D organoids from USH1 patient and control IPSC will provide key insights into the early stages of USH1 phenotype in a human cellular context, while our animal models offer key readouts to monitor USH1 mutations’ impact in an in vivo context. Planned objectives include:
1- Monitor where, when and how inner ear and retinal abnormalities manifest in already available defective mice to establish relevant Usher clinical trial endpoint metrics to weigh therapeutic efficacy.
2- Evaluate and validate in vivo the delivery and efficacy of gene editing via CRISPR/Cas9, aiming to restore normal sensory modalities in available defective mice.
If interested, send a detailed CV and motivated interest as early as possible (Deadline May 15, 2021) to allow sufficient time to prepare the final proposal.

previous job ID: 28534